September 30th - October 4th

9/30-10/04

On the week of October 30th through November 4th, we made LB broth and prepared for transformation of e. Coli. We also did overnight ligation of our kanamycin and pgRNA since in the past, our ligations have failed.


Used was the BioLabs quick ligation procedure.

  • Quick ligation buffer (10ul) was used first as the buffer maintains the PH so the ligase doesn’t kill the pgRNA.

  • 177.8 ng of kanamycin was needed according to the given calculator.

  • 2 ul of 50 ng/u concentration of pgRNA; total of 100 ng needed.

  • 3.5 ul of kanamycin of 50 ng/ul concentration needed for a total of 177.8 ng.

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