October 21st-October 25th

 Week Five (Oct 21-25)

We started the week by gram-staining our pdCas9 and E. coli from 10/21. My first thought was that the actual gram stain result was poor, but I needed to use more ethanol for proper viewing, which was then redone. While I redid this to use more ethanol, we began working on a gel.


The rest of the day was spent discussing microscope techniques along with different types of streaks for innoculation.

  • For a four-way streak, you only pick up your cells ONCE. it’s important to do it quickly and avoid contamination as much as possible. It’s recommended to do it upside down to avoid contamination but, understandably, not everyone is capable of doing it that way, just do your best to avoid contamination and pushing air into the agar which could contaminate


After our inoculation, we put the original plate into the fridge and the new plate in the incubator. We also originally had planned to use one R2A plate and one TGY plate but used two TGY plates as we were all out of our R2A plates.

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