November 25th - November 27th

 Week 10

November 25th - November 27th

After running our big gel, we were left feeling a bit confused as to what the results were. It seems like no digested DNA moved at all, although the non-digested one did move. The ladder ended up showing completely, though. We ultimately decided to come back to this by deciding to choose a smaller DNA fragment that has less base pairs than Lambda DNA does. Since Lambda has such a high number of base pairs (48,502) and the xbar location is so split down the middle, it makes it difficult to see it accurately on our gel. Instead of trying for a third time, we decided to ultimately pick something smaller. We're still in the process of picking out a different type of DNA.

The remainder of the week was spent assisting others with their projects. I worked alongside Vann and gram stained, inoculated, and ran a gel to confirm pRAD (6809 base pairs). We also spent time talking about her project and figured out the details. 

Gel Results

L1: KB Ladder

L2: N/A

L3: Tube 1

L4: Tube 2

L5: Tube 3

L6: Tube 4



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