November 18th-November 22nd

 Week 9 Nov 18- Nov 22nd

Reflecting on last week, we’re at a point where we know that our Lambda DNA did not digest. We had quite a bit of independent time this week and most group members worked on their personal projects, in which I assisted with very briefly. For instance, we did an extraction on 11/19 of E. Coli and pRad that went extremely well.


ng/ul    A260/A280   A260/A230

  1. 350.2     1.88    2.03

  2. 486.2      1.89   2.08


We also did gram stains and plasmid extraction on 11/21 that initially did not go well due to our user error on the very last step. After redoing it, the OD values were still poor. There’s discussion if the actual strain of E. Coli we have is not what we think it is since our plasmid extractions are turning out continuously poor and dirty.


We also decided to redo our big gel on digested lambda, which we had done last time. Last week, when we ran our gel, although we ran it for almost two hours, we were still not one hundred percent certain about if it really digested or not. We decided to redo this gel for essentially the whole lab period at 120 amps and will be discussing the results next week.

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